Understanding the Benefits and Uses of TB-500 (Thymosin Beta-4)

TB-500 main mechanism of action can be broken up into two different parts

The first part of TB-500’s mechanism involves upregulating a cell-building protein known as actin.

Actin facilitates the rejuvenation and upkeeping of cellular architecture, consequently expediting the pace at which tissue injuries recover and wounds undergo self-repair.

Ben Greenfield explains it best in an article describing his experience with TB-500:

“The main functionality of TB500 hinges on the ability to upregulate cell building proteins such as actin, which is a protein that forms (together with myosin) the contractile filaments of muscle cells, and is also involved in motion and metabolism in many other types of cells.

Upregulation of actin allows TB500 to promote healing, cell growth, cell migration and cell proliferation. This not only helps build new blood vessel pathways but also upregulates the type of “good” inflammation that is directly correlated with faster wound healing.”

Put differently, actin plays a pivotal role in achieving peak muscular contraction, elucidating why individuals using TB-500 experience an increased sensation of strength, even without actual increments in muscle size or power.

The second phase of TB-500’s mode of operation encompasses the generation of fresh blood vessels, a process known as angiogenesis. 

This is accomplished by fostering the expression of biomolecules like vascular endothelial growth factor (VEGF), which in turn encourage the maturation of blood cells.

As a result, this leads to heightened blood circulation, arguably constituting the most essential aspect of healing any compromised tissue:

“Angiogenesis is deemed as the most important mechanism for Tβ4-induced cutaneous wound healing and cardioprotection…It is vital for tissue repair as cell survival and regeneration depends on a sufficient supply of nutrients and oxygen.”

Both components collaborate synergistically to bestow the TB-500 peptide with its therapeutic advantages, many of which will be elaborated upon in the following sections.

The entire spectrum of healing possibilities attributed to TB-500 is a direct result of extensive clinical and nonclinical investigations conducted on Thymosin Beta-4.

This encompasses numerous cell culture experiments, a substantial array of animal trials, and a limited but notable count of human clinical trials exploring the health advantages of Thymosin Beta-4. 

Upon a comprehensive review of these studies, it becomes evident that injury healing and wound repair are merely a fraction of the potential attributed to the capabilities of the TB-500 peptide.

A primary pathway through which TB-500 expedites injury recovery involves the reduction in production of pro-inflammatory molecules:

“…administration of 100 micrograms of Tbeta(4) immediately following and at 2 and 4 h after an LD(50) dose of [endotoxin] (60 mg/kg) in mice significantly reduced mortality rates (p< or =0.024) and lowered blood levels of a number of inflammatory cytokines, eicosanoids, and other molecules that are highly elevated following endotoxin administration”.

For those who have been already on a health journey for an extended period, it’s already clear that chronic inflammation stands as the foremost cause behind the persistence and exacerbation of injuries, leading to heightened pain and decreased flexibility. 

However, in conjunction with this, TB-500 also augments the generation of anti-inflammatory compounds:

“TB-4 not only down-regulates inflammatory mediators but also up-regulates preinflammatory cytokine suppressors, such as interleukin-10, and reduces the infiltration and adhesion of inflammatory cells.

Recently, increasing evidence has shown that TB-4 has a strong effect on preventing apoptosis and promoting tissue regeneration”.

Certain researchers even hypothesize that TB-500’s anti-inflammatory characteristics involve multiple mechanisms at work. 

It’s unsurprising that TB-500 has exhibited anti-inflammatory impacts not only in cases of alcohol-induced liver injury but also in the context of rheumatoid arthritis.

TB-500 has been known to protect heart tissue during myocardial infarctions and heart attacks for the past few years:

“In recent reports, due to its activation of resident epicardial progenitor cells and modulation of inflammatory-caused injuries, Tβ4 has been suggested as a promoter of the survival of cardiomyocytes. 

Furthermore, Tβ4 may in skeletal muscle and different organs act in association/synergism with numerous other tissue repair stimulating factors, including melatonin and C-fiber-derived peptides”.

TB-500 can prevent existing heart cells from dying while stimulating the growth of new heart cells.

In a pilot study conducted by Regenerx Biopharmaceuticals, patients who had experienced a heart attack were administered TB-500, and the findings align with the existing corpus of scientific investigation. 

Notably, there was an enhancement in heart function, the absence of adverse events, and successful repair and regeneration of the compromised heart tissue.

Fortunately, TB-500’s aptitude for rejuvenating impaired cells was discerned as far back as 1999, when a dermal study conducted on rats demonstrated the peptide’s ability to “facilitate wound closure, enhance angiogenesis, and expedite collagen deposition.”

Organs: Beyond effectively regenerating cardiac tissue, TB-500 exhibits potential in addressing liver fibrosis (characterized by excessive scarring leading to liver dysfunction), kidney injury caused by acute ischemia (resulting from inadequate blood flow to the kidney), and lung injury induced by acute ischemia.

Skin Injuries: In a particular study, burn wounds in diabetic mice were effectively healed using TB-500, while another study involving mice revealed TB-500’s ability to facilitate complete healing of deeply penetrating “full-thickness cutaneous wounds,” all while minimizing scarring.

Ligaments: A rat study demonstrated that TB-500 administered as a single treatment enabled rats with severed ligaments to regain biochemical functionality and experience uniform regeneration of collagen fibers.

Tissue: Notable Phase II clinical trials involving the utilization of TB-500 to address pressure and stasis ulcers showcased a 30-day acceleration in healing, coupled with reduced inflammation and prevention of re-infection.

Pain Alleviation: An investigation into electroacupuncture tolerance in rats unveiled that the administration of TB-500 resulted in diminished perceptions of pain.

If you’re grappling with an injury that’s displaying prolonged or non-existent healing, exploring a well-structured dosing regimen involving the TB-500 peptide could prove to be a worthwhile consideration!

TB-500 contributes to fortifying the immune system through various mechanisms, including “facilitating the progression of B cells to plasma cells for antibody production… influencing lymphocyte maturation and differentiation… and modulating immunity.”

Certain peptide experts even advocate for combining TB-500 with an immune enhancer like Thymosin Alpha-1 to achieve more favorable outcomes.

Researchers from the Oak Ridge National Lab in the United States have even postulated that elevated concentrations of Thymosin Beta-4 in women infected with coronaviruses (twice that of men) might elucidate why their symptoms tend to be less severe.

A plausible rationale behind this phenomenon could be linked to the site of Thymosin Beta-4 production:

“The thymus gland is critical in developing a healthy immune system in childhood. 

It stimulates the production of white blood cells into T cells and develops crucial defenses against autoimmunity (when the immune system attacks the body). 

TB4 is vital for the development and production of lymphocytes into T cells and is a crucial part of the immune and endocrine systems”.

Might TB-500 possess the potential to repair brain damage? 

The notion holds a technical plausibility, given that the brain constitutes an individual organ. 

A patent attributed to RegeneRx Biopharmaceuticals hints at the possibility of TB-500 addressing peripheral neuropathy symptoms by targeting the underlying cause of nerve cell damage.

A notable application of TB-500 could be its utilisation in cases of traumatic brain injury (TBI). This prospect is gaining traction as a significant therapeutic avenue following highly promising outcomes from a groundbreaking rat study, in which recovery commenced as early as 6 hours after treatment initiation.

Further investigations involving mice demonstrate that TB-500 can yield enhancements in functional behaviors, facilitate the growth of new blood vessels in damaged brain regions, and potentially reinstate sensory-motor abilities!

The persistent issue of dry eye disorder was resolved with the emergence of TB-500:

“Thymosin β4 (Tβ4), a small, naturally occurring protein, promotes complete and faster corneal healing than saline alone or prescription agents (doxycycline and cyclosporine) in various animal models of eye injury. 

In human trials, it improves both the signs and symptoms of moderate to severe dry eye with effects lasting beyond the treatment period.”

An example of such a human trial was a Phase II clinical study that verified the safety and efficacy of TB-500 in significantly alleviating the discomfort linked to severe dry eye, even after a lapse of 4 weeks since the last dosage.

The remarkable attributes of TB-500, encompassing wound healing, cellular migration, inhibition of cell death, and anti-inflammatory effects, exhibit equally notable results within the cornea of the eye!

After reading our science backed article onTB-500, we know you will be raving to give it a try, especially if you’re just discovering Peptide Therapy.

Across numerous conducted clinical trials, both healthy and ailing adult participants have administered TB-500 without encountering any instances of toxicity or adverse reactions. 

Moreover, although there is a dearth of published studies involving human subjects, anecdotal evidence suggests that any potential side effects are generally mild and transient, often subsiding swiftly after a few administrations.

You can acquire any of our peptides by booking in a consultation with our Integrative and Functional Medicine practitioner, Jessica, who has years of experience working with Peptide Therapy.

These consultations are available for international clients, and are not subject to the UK only.  

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